Oil immersion is a microscopy technique used to increase the magnification and resolution of high-power objective lenses, typically 100×, for observing fine details in specimens like bacteria, blood cells, or tissue sections. A special immersion oil with a refractive index similar to glass is applied between the objective lens and the cover glass, reducing light refraction and allowing more light to enter the lens.
Key Points:
Enhances clarity and resolution at high magnifications.
Requires immersion oil specifically designed for microscopy.
After use, lenses and slides must be cleaned carefully to avoid residue or damage.
Commonly used in microbiology, hematology, and pathology labs.